IB47010 Gel/PCR DNA Fragment Extraction Sample Kit for 4 Preparations

The Gel/PCR DNA Fragments Extraction Kit was designed to recover or concentrate DNA fragments (100 bp-10 Kb) from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column (1). Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer or TE. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are up to 90 percent for Gel Extraction and up to 95 percent for PCR Clean up. The entire procedure can be completed in 20 minutes and the eluted DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation. For users who require a higher recovery from small base pair DNA fragments (50-200 bp) or large base pair DNA fragments (greater than 8 Kb). The quality of the Gel/PCR DNA Fragments Extraction Kit is tested on a lot-to-lot basis by isolating DNA fragments of various sizes from either aqueous solutions or agarose gel. The purified DNA is checked by electrophoresis. Caution: DF buffer contains guanidine thiocyanate which is a harmful irritant. During operation, always wear a lab coat, disposable gloves, and protective goggles. Operation Time: 15 min for PCR clean-up 20 min for gel extraction. 20-50 microliter elution volume.

• Kit contains (4) DF columns, (4) 2mL collection tubes, 3mL DF buffer, 1mL wash buffer (4mL add ethanol) and 1mL elution buffer
• Sample: up to 300mg agarose gel slice and up to 100 microliter PCR product or other enzymatic reaction
• Spin column format; centrifuge/Vacuum manifold operation
• 10 microgram DNA binding capacity; expectant yield is 80-90 percent for gel extraction and 90-95 percent for PCR clean-up
• Applications: PCR fluorescent or radioactive sequencing, restriction digestion, DNA labeling, ligation